Effects of β-caryophyllene on behavior and synaptic plasticity-related proteins in depressed mice
HUA Yi
WANG Yunxue
YANG Wanling
LIN Songyang
LI Siyi
DENG Xianqing
Abstract：Objective:To study the effect of β-caryophyllene on the behavior and the expression levels of synaptic plasticity-related proteins in depressed mice.Methods:The mice were randomly divided into blank control group,model group,fluoxetine group(10 mg/kg)and low,medium and high dose groups(50、100、200 mg/kg)of β-caryophyllene.The depressed mice model was established by chronic unpredict-able mild stress.At the same time of modeling,the corresponding drugs were given by gavage once a day for 6 weeks.Detect changes in body weight of mice in each group.Evaluation of depression levels through forced swimming and open field behavior experiments.HE staining was used to observe the morphology of hippocampus.ELISA was used to detect the content of SYP,GDNF and NGF in the hippocampus.Immu-nofluorescence was used to detect the expression level of MAP2 in the hippocampus.Result:Compared with the blank control group,the body weight in the model group mice decreased(P<0.01),the forced swimming immobility time increased(P<0.01),the total distance,average speed,and number of stands during open field test were decreased(P<0.05,P<0.01),the morphological and structural of the hippo-campus is significantly damaged,the expression levels of SYP,GDNF,NGF and MAP2 in the hippocam-pus were decreased(P<0.01).Compared with the model group,the body weight in each dose group of β-caryophyllene increased(P<0.05,P<0.01),the forced swimming immobility time decreased(P<0.01),the total distance,average speed,and number of stands during open field test were increased(P<0.05,P<0.01),the morphological and structural damage of the hippocampus has been significantly im-proved,the expression levels of SYP,GDNF,NGF,and MAP2 in the hippocampus were increased(P<0.05,P<0.01).Conclusion:β-caryophyllene has antidepressant effect,and its mechanism may be relat-ed to increasing the expression levels of synaptic plasticity related-proteins and improving hippocampal synaptic plasticity damage.
Keywords：β-caryophyllenedepressionsynaptic plasticityhippocampus
Publication Date：2025-08-01
Online Publishing Date：2026-08-28(First online date of this platform, not the publication date of the document)
Pages：10( 596-605 )