Bio-Synthesis Progress of Ursodeoxycholic Acid
ZHANG Xue
ZHANG Yue
LIU Mengyu
LUAN Haoni
SONG Peng
ZHOU Zhengsong
WANG Fei
XU Wei
Abstract：Ursodeoxycholic acid(UDCA)is a fundamental medication in the treatment of hepatobiliary dis-eases and used as an adjuvant in the treatment of certain cancers and neurological disorders.The chemical synthesis method of UDCA is environmentally unfriendly,low yields output and cannot be fully met the production demand yet.In contrast,the biosynthesis of UDCA is characterized by high conversion rates,low energy conservation,and environmental friendliness.At present,it represent the direction of research and development for UDCA synthesis.Availabled substrates are utilized for biosynthesis such as chenode-oxycholic acid(CDCA),cholic acid(CA),or lithocholic acid(LCA).substrates are catalyzed by four en-zymes:7alpha-hydroxysteroid dehydrogenase(7a-HSDH),7beta-hydroxysteroid dehydrogenase(7β-HS-DH),Lactate dehydrogenase(LDH),and Glucose dehydrogenase(GDH).There are two methods for syn-thesizing UDCA.It includes free enzyme catalysis and whole-cell synthesis.Free enzyme catalysis method are subdivided into"one-pot two-step"and"one-pot one-step".Whole-cell catalysis uses engineered cells as reaction vessels.The synthesis of UDCA is catalyzed by enzyme systems of cell.This paper reviews This article gives an overview of the enzymes involved,synthesis methods and the progress in synthesis technology for both free enzyme catalysis and whole-cell synthesis of UDCA.It provides technical refer-ences for the research and production of UDCA.
Keywords：ursodeoxycholic acidbiological synthesisfree enzyme-catalyzed synthesiswhole-cell syn-thesis
Publication Date：2024-04-28
Online Publishing Date：2026-08-28(First online date of this platform, not the publication date of the document)
Pages：10( 101-110 )