Isolation,Identification and Degradation Pathway Study of a 3,5,6-trichloro-2-pyridinol Degrading Strain Delftia sp. DFT
JIA Nan
YUE Caixu
LÜ Xueru
WANG Xueting
DING Ning
WANG Shenghui
Abstract：Microbial degradation is an effective method to eliminate 3,5,6-trichloro-2-pyridinol(TCP).However,there is still no detailed information on the mirobial metabolic mechanism of TCP.In order to explore the complete degradation pathway and metabolic mechanism of TCP.A TCP degrading strain was isolated from farmland soil.The degradation ability and the metabolites of TCP was detected by high per-formance liquid chromatography(HPLC)and liquid chromatography-mass spectrometry(LC-MS),re-spectively.The TCP degrading strain was identified as Delftia sp.DFT which could degrade 50 mg/L of TCP at a rate of 34.28％within 48 hours.This strain could also degrade other chloropyridine compounds such as 5-dichloro-2-pyridinol,6-chloro-2-pyridinol and 2-hydroxypyridine.Twelve TCP intermediate me-tabolites were detected in strain DFT and two possible degradation pathways of TCP were proposed on the basis of LC-MS analysis.The hydrolyzation-oxidation dechlorination pathway,the reduction dechlorina-tion pathway,the denitrification pathway and the alkylation pathway might be involved in TCP biodegra-dation by strain DFT.This is the first report on four different pathways responsible for TCP degradation in the genus Delftia sp.,which provides new information for the study of TCP degradation mechanism in pure culture.
Keywords：356-trichloro-2-pyridinoldegrading bacteriaisolation and identificationDelftia sp.deg-radation pathway
Publication Date：2024-04-28
Online Publishing Date：2026-08-28(First online date of this platform, not the publication date of the document)
Pages：12( 89-100 )