Identification and Functional Study of Cyclodextrin Glycosyltransferase JSL-1
LI Ru
DING Xinjie
SHANG Jinhai
QIN Linxue
WEI Kai
WANG Yue
LI Pan
Abstract：Cyclodextrin glycosyltransferase(CGTase)can catalyze starch and its derivatives to produce cy-clodextrin(CDs)through cyclization reaction.In this study,the cyclodextrin glycosyltransferase JSL-1 protein in Bacillus toyonensis P18 was successfully obtained by means of genetic engineering technology to explore its product specificity and enzymatic properties.Meanwhile,the JSL-1 gene was overexpressed in-to Nipponbare rice to study the effect of cyclodextrin glycosyltransferase JSL-1 on rice starch quality.The results showed that JSL-1 mainly catalyzed starch to produce α-CD,and its optimum reaction pH and tem-perature were 8.0 and 50℃,respectively.With the increase of JSL-1 expression level,the amylose con-tent and aging degree in rice decreased,and the light transmittance and freeze-thaw stability increased,which was beneficial to improve the eating quality and appearance quality of rice.In conclusion,the study of cyclodextrin glycosyltransferase JSL-1 is beneficial to the improvement of cyclodextrin production tech-nology and the improvement of rice quality.
Keywords：cyclodextrin glycosyltransferaseproduct specificityenzymatic propertiestransgenic ricestarch characteristics
Publication Date：2024-04-28
Online Publishing Date：2026-08-28(First online date of this platform, not the publication date of the document)
Pages：9( 80-88 )