The Extraction and the TLC Analysis of Flavonoids f rom Leaves of M alus M icromalus (Rosaceae)
QIAN Ke-cheng
LIU Lian-fen
Abstract：To investigate the kinds of flavonoids in leaves of Malus micromalus Makino ,flavonoids was colored by the method of aluminum nitrate and sodium nitrite colorimetric ,and the content of fla‐vonoids was determined by spectrophotometry ,the optimized extraction system of total flavonoids in M . micromalus was thus determined by the means of orthogonal experimental design .And then ,flavonoids purified by ethyl acetate or petroleum ether were analyzed by microemulsion TLC .The results showed that the most proper fators to extract total flavonoids from leaves of M .micromalus were :70% ethanol extracting for 4 h ,at the extraction temperature of 80℃ ,in the extracting system with solid :liquid=1 :30 .As TLC shown ,the extraction of ethyl acetate contains many kinds of flavonoids ,3 of them are rutinum ,quercetin and puerarin .
Keywords：Malus micromalusflavnoneextractionTLC
Publication Date：2015-01-01
Online Publishing Date：2026-08-28(First online date of this platform, not the publication date of the document)
Pages：4( 46-49 )