Effects of miRNA hsa-let-7b-5p on FAS expressions and apoptosis of HUVECs in high glucose state

FANG Jie; JIN Qun; HAN Shufang; TAN Hong; LI Bin; LIU Fang

doi:10.3969/j.issn.2097-1753.2025.01.008

Abstract：Objective To investigate the effect of miRNA hsa-let-7b-5p on factor-related apoptosis(Fas)expressions and apoptosis of human umbilical vein endothelial cells(HUVECs)in high glucose state.Methods HUVECs were cultured and divided into the control group(at the medium glucose concentration of 5.5 mmol/L),high glucose group(at the medium glucose concentration of 33.0 mmol/L),high glucose+miRNA hsa-let-7b-5p mimic group(at the medium glucose concentration of 33.0 mmol/L+miRNA hsa-let-7b-5p mimic transfected cells),and high glucose+miRNA hsa-let-7b-5p inhibitor group(at the medium glucose concentration of 33.0 mmol/L+miRNA hsa-let-7b-5p inhibitor transfected cells).The proliferation viability of the four groups was detected by CCK8 assay,the protein level of Fas in each group of cells by Western blotting,and the mRNA levels of miRNA hsa-let-7b-5p and Fas in HUVECs by RT-PCR.TUNEL staining was performed to detect the apoptosis of cells in each group.Results The miRNA hsa-let-7b-5p mimic transfection increased the proliferative activity(P＜0.001)but decreased the apoptotic rate(P＜0.001)of the cells.The expression levels of Fas mRNA(P＜0.001)and Fas protein(P＜0.001)were decreased while the expression level of hsa-let-7b-5p mRNA was increased(P＜0.001).The miRNA hsa-let-7b-5p inhibitor transfection decreased the proliferative activity(P＜0.001)but increased the apoptotic rate(P＜0.001)of the cells.The expression levels of Fas mRNA(P＜0.001)and Fas protein(P＜0.001)were increased while that of hsa-let-7b-5p mRNA was decreased(P=0.001).Conclusion In high glucose state,miRNA hsa-let-7b-5p may inhibit apoptosis of HUVECs and protect vascular endothelial cells against atherosclerosis.