Expressions of GRP78 and ATF4 in peripheral blood of postmenopausal patients with osteoporosis and their clinical significance
TIAN Lijun
GUO Yusong
WANG Xiaoyan
GUO Zheming
Abstract：Objective To explore the levels and clinical significance of peripheral blood glucose-regulated protein 78(GRP78)and activating transcription factor 4(ATF4)in patients with postmenopausal osteoporosis(PMO).Methods A total of 96 PMO patients admitted to the Third Hospital of Shijiazhuang between December 2018 and May 2021 were selected as the PMO group while another 96 menopausal women confirmed to be healthy by physical examinations served as the normal group.The basic data of the two groups was compared.Enzyme linked immunosorbent assaywas used to measure the serum levels of GRP78 and ATF4 while electrochemiluminescence immunoassay was adopted to measure the serum levels of β-Crosslaps and procollagen Ⅰ type N-terminal propeptide(PINP).The dual-energy X-ray bone densitometer was used to detect lumbar bone mineral density(BMD)while the Pearson method was employed to analyze the correlations between serum levels of GRP78 and ATF4 in PMO patients and β-Crosslaps,PINP and BMD as well as those between serum levels of GRP78 and ATF4 in PMO patients.Logistic regression was used to analyze the influencing factors of PMO.Results The waist-to-hip ratio and BMD of the PMO group were lower than those in the normal group(t=9.76,15.47,both P＜0.001),but the serum levels of GRP78,ATF4,β-Crosslaps and PINP were higher(t=18.32,16.81,8.15,14.84,all P＜0.001).The serum levels of GRP78 and ATF4 in PMO patients were positively correlated with β-Crosslaps and PINP(r=0.56,0.48,0.48,0.52,P＜0.001,＜0.001,＜0.001,=0.006),but negatively with BMD(r=-0.54,-0.44,both P＜0.001).The serum level of GRP78 of PMO patients was positively correlated with ATF4(r=0.595,P＜0.001).PINP,GRP78,β-Crosslaps and ATF4 were risk factors for PMO(OR=2.518,2.672,2.271,2.336,all P＜0.001),while BMD was a protective factor(OR=0.583,P＜0.001).Conclusion The serum levels of GRP78 and ATF4 in patients with PMO are relatively high,which are related to β-Crosslaps,PINP,and BMD.GRP78 and ATF4 may be potential targets for the diagnosis and treatment of PMO.Determination of serum GRP78 and ATF4 levels is helpful for clinical prevention and treatment of PMO.
Keywords：Glucose-regulated protein 78Postmenopausal osteoporosisβ-CrosslapsActivating transcription factor 4Procollagen Ⅰ type N-terminal propeptide
Publication Date：2024-02-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 42-46 )