Long non-coding RNA Florpar induces apoptosis in cerebrovascular smooth muscle cells in simulated microgravity rats
SONG Jibo
CHEN Li
ZHANG Bin
BAI Yungang
XIE Manjiang
Abstract：Objective To investigate the effects of long non-coding RNA Florpar on cerebrovascular remodeling of simulated microgravity rats.Methods A high throughput microarray assay was performed to screen the changes of LncRNA expressions in cerebral arteries of 4-week hindlimb unweighting rats.Aortic smooth muscle cells A7r5 were transfected with LncRNA-Florpar plasmid or siRNA respectively and the apoptotic rate was examined by Hoechst+PI staining.The expressions of apoptosis-related proteins(Bcl-2 and Bax)were also assessed by Western blotting.Results Compared with control rats,the expression of LncRNA-Florpar was significantly increased in cerebral arteries of rats that had undergone 4-week simulated microgravity.Furthermore,upregulation of LncRNA-Florpar significantly increased the apoptotic rate and the ratio of Bax/Bcl-2 in A7r5 cells.In contrast,downregulation of LncRNA-Florpar significantly decreased the apoptotic rate and the ratio of Bax/Bcl-2 in A7r5 cells.Conclusion We have found for the first time that the expression of LncRNA-Florpar can be significantly increased in cerebral arteries of simulated microgravity rats.Furthermore,LncRNA-Florpar can induce apoptosis in vascular smooth muscle cells.The present study indicates that LncRNA-Florpar can sense the change of mechanical signals in a microgravity environment and then participate in cerebral arterial remodeling by regulating the apoptosis of VSMCs.
Keywords：simulated microgravitylong non-coding RNAcerebral arteryapoptosis
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 145-149 )