Protective effects of amniotic mesenchymal stem cells on early kidney injury of rats induced by severe burns

MOU Huan; MA Ping; ZHOU Xinli; QIANG Tinghui; DU Xingguo; DING Chen; XUE Chao

Abstract：Objective To explore the effect and mechanism of amniotic mesenchymal stem cells on rats' early kidney injury induced by severe burns. Methods Fat tissue was isolated and cultured from rats, and trypsin-collagenase digestion method was used to culture rat AMSCs. The surface marker of AMSCs was assayed with flow cytometry. Thirty-six male SD rats were divided into sham group, burn group and AMSCs treatment group with the random number table. Rats were sacrificed and kidney tissue and abdominal aortic blood were collected after 48 hours. Serum creatinine and urea nitrogen were assayed with ELISA kit. Caspase-3, TNF-α, IL-1β and IL-10 mRNA levels were detected by fluorescence RT-PCR. Results AMSCs cultured in vitro were detected by flow cytometry after three passages. Positive rates of CD29, CD44, CD90, CD105 and CD34 were 96.4％、89.8％、94.2％、98.3％ and 2.1％ respectively. Serum creatinine and urea nitrogen levels of burn group were higher than sham group (P<0.05). AMSCs treatment group had a lower level of serum creatinine and urea nitrogen than burn group (P<0.05). Caspase-3, TNF-α, and IL-1β mRNA levels in the kidney of burn group were higher than in sham group (P<0.05). In AMSCs treatment group, the levels of caspase-3, TNF-α, and IL-1β mRNA were all lower than in burn group (P<0.05). However, the IL-10 level was significantly higher than in sham or burn group (P<0.05). Conclusion AMSCs can decrease serum creatinine and urea nitrogen levels, reduce apoptosis of kidney cells, inhibit the expression of such inflammatory factors as IL-1β and TNF-α, and promote the expression of the anti-inflammatory factor IL-10, thus having protective effects on early kidney injury of rats induced by severe burns.