miRNA-138-5p inhibits melanoma cell proliferation by targeting CCNE1

QIAO Li; YANG Zhiyong; LI Xinji; LIU Wen

Abstract：Objective To study the role of miRNA-138-5p in the proliferation of melanoma cells and the mechanism. Methods MTT assay was used to detect the proliferation of melanoma cells after miRNA-138-5p transfection. Real-time PCR was used to detect the changes of the target gene CCNE1 mRNA after miRNA-138-5p transfection. Western blot was used to detect the changes of CCNE1 protein level after miRNA-138-5p transfection. Luciferase reporter assay was used to detect the effect of miRNA-138-5p on CCNE13's untranslated region (3'UTR) and its mutations. MTT assay was used to detect the effect of co-transfection of miRNA-138-5p and CCNE1 on the proliferation of melanoma cells. Results Overexpression of miRNA-138-5p inhibited the proliferation of melanoma cells. CCNE1 was a functional target gene of miRNA-138-5p. Transfection of miRNA-138-5p could reduce the mRNA and protein levels of CCNE1 in cells. Co-transfection of miRNA-138-5p and CCNE1 could partially eliminate the effect of miRNA-138-5p on the proliferation of melanoma cells. Conclusion miRNA-138-5p plays an important role in the proliferation of melanoma cells through its target gene CCNE1, and miRNA-138-5p can be taken as a key drug target in melanoma cells.