Effects of 5-ALA-PDT on proliferation and apoptosis of A431 cells

QIAO Li; LIU Yan; LI Yonghe; LIU Wei

Abstract：Objective To analyze the effect of 5-ALA PDT on the proliferation and apoptosis of the squamous cell carcinoma A431.Methods Cells were divided into control group and treatment group at different doses of 5-ALA-PDT (5-ALA incubation concentration was 0.05, 0.1, 0.2, 0.4, 0.8 mg/ml respectively, and the laser energy density was 1.25, 2.5, 5 J/cm2 respectively). After 24 h treatment, MTT method was used to test the effect of 5-ALA-PDT on the survival of A431 cells, while the flow cytometry method (FCM) was used to test the effect of 5-ALA-PDT on A431 cells' cycle and apoptosis.ResultsThe result of MTT method showed that when A431 cells were treated by 5-ALA of different incubation concentrations (0.05, 0.1, 0.05, 0.1, 0.05 mg/ml) and of different laser energy densities (1.25, 2.5, 5 J/cm2), the killing effect of A431 cells by 5-ALA-PDT was positively related to the incubation concentration and laser energy density. But when the incubation concentration of 5-ALA reached 0.8 mg/ml, there was no obvious difference (P>0.05) in the survival rate of cells treated at different laser energy densities. The flow cytometry method (FCM) test results showed that after 5-ALA-PDT treatment, A431 cells were stranded in the G0/ Gl period, while the proliferation index (PI) of A431 cells was reduced. The Annexin V-FITC-PI double dyeing results showed that compared with the control group, the apoptosis and necrosis rate of A431 increased significantly (P<0.05) after 5-ALA-PDT treatment.Conclusion5-ALA-PDT treatment can inhibit the proliferation of skin squamous cell carcinoma A431 cells, but promote the apoptosis of A431 cells.