Production of NF-κB, VAP-1 and IL-6 inhibited by Ginkgo biloba extract in endothelial cells
SHI Erlan
LI Zhongdong
SUN Jian
GOU Xuerui
WANG Jianchang
Abstract：ObjectiveTo establish an oxidative stress model using HCAEs stimulated by activated platelets in vitro so as to explore whether Ginkgo biloba extract can inhibit the phosphorylation of NF- kappa B the production of interlukin-6 (IL-6) and vascular adhesion protein (VAP-1) in HCAECs, stimulated by activated platelets.MethodsActivated platelets were used to stimulate HCAECs to establish the oxidative stress model before, Ginkgo biloba extract (4, 40 or 400μg/ml) was used to act on them. Cell. culture supernatant was collected to measure the amount of IL-6 and VAP-1 via enzyme linked immune sorbent assay (ELISA), and total protein was extracted to examine the expression of NF-κB PP65 by Western Blot.ResultsThe results showed that the secretion of target molecules was increased significantly after HCAECs were stimulated by activated platelets. EGb at a therapeutic dose can inhibit the expression of p65, PP65 and the generation of IL-6,VAP-1, in a dose-dependent manner compared with activated platelets group (P<0.05).ConclusionEGb can attenuate the generation of IL-6,VAP-1 by inhibiting the expression of p65, PP65 significantly in HCAECs stimulated by activated platelets.
Keywords：Ginkgo biloba extractInterlenkin-6Vascular adhesion protein lNF-κBp65PP65
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 242-245,251 )