Effects of sustained high positive acceleration on structure and HSP70 mRNA expression of tongue striated muscle in SD rats

YIN Yin; CHEN Xin; ZHOU Xiaoling; LI Dongxia; FENG Yan; WANG Zhe

Abstract：Objective To investigate the possible influence on high +Gz exposure on structure and heat shock protein 70(HSP70)mRNA relative expression in tongue striated muscle of SD rats, and to observe the effects of depside salt from salvia miltiorrhiza on high +Gz exposure.Methods A total of 27 male SD rats were randomly divided into 9 different groups: negative control group, +5 Gz positive control group, +10 Gz positive control group, +5 Gz positive treatment groups(high, middle and low dose), +10 Gz positive treatment groups(high, middle and low dose), with 3 rats in each group respectively. The rats in positive control and drug groups were fixed with rats kept-keeling devices for centrifugation exposed to +5 Gz and +10 Gz, the rats in drug groups were injected into abdomen with different doses depside salt from salvia miltiorrhiza 30 min prior to +Gz exposure, the rats in other groups were injected with the same value normal saline. The tongue tissues were observed by histological method through light microscope, and real time qPCR was applied to determine the relative expression of HSP70 mRNA.Results The normal structure of tongue striated muscle was observed in the negative control group, fibers of striated muscle for other groups were basically normal, with sparse for intercellular substances occasionally, cell arrangement disorder and muscle texture indistinctly. Compared to the negative control group, the relative expression of HSP70 mRNA significantly higher and increased with the trend of raising +Gz value in positive control groups. Compared to the positive control groups, the relative expression of HSP70 mRNA decreased in the treatment groups.Conclusion+Gz may cause injury to the tongue striated muscle through increasing relative expression of HSP70 mRNA, and the injury extent was related to +Gz raising. Depside salt from salvia miltiorrhiza can inhibit HSP70 mRNA expression on +Gz exposure and have protective effects on tongue tissue on +Gz condition.