Human vascular endothelial cells express TMEM16B speciifcally
FU Zhaojun
LIU Hongjin
CHENG Jun
XU Xianrong
DAVID J.Beech
Abstract：ObjectiveTo investigate the expression of TMEM16A and TMEM16B protein in human vascular endothelial cells and try to clarify the function of TMEM16B.MethodsReal time PCR was used to check the expression of TMEM16A and TMEM16B in human umbilical vein endothelial cells (HUVECs). Western-Blot and immunofluorescence methods were used to confirm the expression of TMEM16B in HUVECs. Flex-station was performed to detect the change of intracellular calcium in HUVECs marked with fura-2am.ResultsHUVECs expressed TMEM16B but not TMEM16A. Western-Blot and immunofluorescence methods both proved that HUVECs expressed TMEM16B. After being intervened by siRNA of TMEM16B, with the stimulation of 50 nM VEGF, HUVECs in solution with normal Ca2+ showed not only an increased peak value but also an advanced latency of peak value. Above effect remained when HUVECs were in solution without Ca2+.ConclusionHUVECs expressed TMEM16B specifically,and it could play an negative feedback function of intracellular calcium releasing evoked by VEGF in physiological condition.
Keywords：Vascualr endothelial cellsTMEM16BIon channelsIntracellular calcium regulation
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 151-153,157 )