Neuroactive substances protein expression in rat spinal trigeminal nucleus caudal subnucleus and trigeminal ganglia under sustained high acceleration

LI Hongshi; FENG Yan; YIN Yin; CAO Junkai; CHEN Xin

Abstract：ObjectiveTo investigate the possible role of substance P(SP), N-methyl-D-aspartate receptor antagonists(NMDAR)in rat spinal trigeminal nucleus caudal subnucleus(Vc) and ppTA,β-calcitonin-gene-related peptide(β-CGRP), PN3, NaN in the trigeminal ganglion(TG) under repeated +Gz condition.Methods 36 male SD rats were divided into control group(N), +5 Gz group(5 G), +10 Gz group(10 G) randomly. The rats in each group were fixed in the experiment with small animal centrifuge. Process simulation in different + Gz numerical conditions. +5 Gz borne +5 Gz group for 5 minutes(onset rats about 0.5 G/s, 5 times/d with +1 Gz for 1 minute, each for 30 s, with intervals 4 d/wk, 3 weeks in total). +10 Gz group was repeatedly exposed to condition +10 Gz(onset rats about 0.5 G/s, 5 times/d with +1 Gz for 1 minute intervals, each for 30 s, with intervals 4 d/wk, lasting 3 weeks in total). Control group rats were in accordance with the same time and frequency, only in the centrifuge the same fixed device remain fixed for 5 min, did not do any sustained +Gz centrifuge experiment intervention. TD and SpVc were removed after +Gz examination. After the total protein extracted, the expression of SP, NMDAR, ppTA,β-CGRP, PN3 and NaN were detected by Western-Blot analysis.ResultsCompared with the control group, the +5 Gz group and the +10 Gz group expressions of SP, NMDAR, ppTA,β-CGRP, PN3 and NaN were significantly up-regulated(P<0.05).ConclusionIn the +Gz environment, the biochemical characteristics of the primary sensory neurons were significantly changed, and the active substance in the TMD was very important in the pain transmission. These indicate that the above-mentioned neural active substances is closely related to the conduction mechanism of the peripheral and central nervous system in +Gz environment.