Study on the molecular mechanism of high +Gz induced brain injury and the protection of low +Gz preconditioning
WU Feng
CHEN Liang-en
ZHAO An-dong
GE Hua
LI Xia
ZHAN Hao
Abstract：ObjectiveTo identify differentially expressed genes in hippocampus of rats with high +Gz stress exposure and low +Gz preconditioning,so as to further understand the molecular mechanisms of positive acceleration induced brain injury. MethodsMale SD rats were randomly assigned to three groups: control group, +10 Gz stress group and low +Gz preconditioning group. Total RNA was prepared from experimental rats hippocampus after 24 hour of +10 Gz exposure. The method of microarray was applied to detect the differentially expressed genes and analyze the pathways through KEGG database.ResultsThe results showed that there were 846 genes differentially expressed in +10 Gz stress group and 992 genes in low +Gz preconditioning group compared with the control group. There were 56 KEGG pathways in +10 Gz stress group, 49 KEGG pathways in low +Gz preconditioning group.ConclusionsThe results indicated that the differentially expressed genes were involved in different signaling pathways. These patterns of gene expression might contribute to understanding the molecular mechanism of high +Gz stress-induced brain injury and its countermeasures.
Keywords：Acceleration stressGene microarrayHippocampusRat
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 185-188 )