Biological monitoring and analysis of the endoscope room

LI Xiao-bao; WU Qiong; LIU Yan-feng; GUO Wen-tao; ZHAI Hong-yan

Abstract：Objective We intended to analyze the changes in the number of colonies, compared before with after endoscopic disinfection and surface microbial load, the cleaning and disinfection of endoscopy room links, and to provide an objective evaluation. Method The detection of total bacterial number in digestive endoscope before and after acidified water disinfection was made to compare the biological clearance while the content of ATP in pools, drying station, faucets, water guns and air guns was detected by fluorescence assay ATP to compare its biological clearance. Results The contamination of gastrocopy and entercopy was most serious, the mean total bacteria colong was 1 785.8 CFU/100 cm2 and 2 369.3 CFU/100 cm2 respectively; after acidified water disinfection, the clearance rate of gastrocopy and entercopy was 100% and 99.9% respectively. The contamination of pools, drying station, faucets, water guns and air guns was dramatically different. The mean value of relative light unit (RLU) of ATP above-mentioned five kinds of surface was 139.4, 10.6, 753.8, 229.0, 5.8 respectively, and the clearance rate was 84.8%,90.2%,89.2%,89.9% and 90.3% respectively. Conclusion After acidified water disinfection, the microbial load of gastrocopy and entercopy meet the requirements; the mean highest value of RLU is the faucets, the mean highest microbe removal rate is the air gun, and the lowest is the pools.