Effect of salidroside on UVB-irradiated HaCaT cells by upregulating the activity of Nrf2

YUANXiao-Ying; MAHui-min; SHAOLi-fang

Abstract：Objective To investigate the effect of salidroside on Ultraviolet B(UVB)-irradiated HaCaT cells by upregulating the activity of Nrf2. Observe the change of MDA and GSH. Methods Cells were divided into five groups: the cells of G1 untreated, the cells of G2 given UVB irradiation with 100 mJ/cm2; the cells of G3, G4 and G5 added with 20, 40μg/ml and 80μg/ml salidroside respectively 24 hours before given UVB irradiation with 100 mJ/cm2. 24 hours later the HaCaT cells were analyzed by immunocytochemistry to observe Nrf2 nuclear translocation. The Levels of MDA and GSH were measured by spectrophotometric method. Results Salidroside, especially of 40μg/ml and 80 μg/ml salidroside, resulted in Nrf2 nuclear translocation significantly. Compared with G1, the levels of MDA increased significantly(P<0.01) and the levels of GSH decreases significantly in G2 (P<0.005). However, the levels of MDA in 40μg/ml and 80μg/ml salidroside treated group decreased significantly compared with G2 (all P<0.05). The levels of GSH in 40μg/ml and 80μg/ml salidroside treated group increased significantly compared with G2 (all P<0.01). Conclusion Salidroside may reduce the oxidative damage by UV radiation in HaCaT cells through enhanced Nrf2 nuclear translocation, reduced the level of MDA and raised the level of GSH.