Effect of haploidentical hematopoietic stem cell transplantation with mesenchymal stromal cells infusion in patients with aplastic anaemia following delayed graft rejection after HLA-matched unrelated donor transplant
YANGShuang
WANGSheng
ZHENGXiao-li
DINGLi
WANGZhi-dong
YANHong-min
WANGHeng-xiang
Abstract：Objective This study was aimed to observe the effectiveness of co-infusion of mesenchymal stromal cell and hematopoietic stem cell in the treatment of the delayed rejection of HLA-matched transplant. Method Two cases of aplastic anemia who received HLA-matched unrelated HSCT were diagnosed as delayed graft rejection that occurred 2 and 3 months post transplantation respectively. The patients received a conditioning regimen consisting of busulfan, fludarabine and cyclophosphamide. Anti-thymocyte globulin or anti-CD52 antibody was used to deplete T lymphocytes in vivo. Graft-versus-host disease prophylaxis was conducted with cyclosporine A, methotrexate, mycophenolate mofetil, and anti-CD25 monoclonal antibody. The patients received grafts including G-CSF stimulated bone marrow, peripheral blood stem cell and umbilical cord mesenchymal stromal cell. Results Two patients had rapid hematopoietic engraftment, and DNA fingerprint analysis revealed complete donor chimera. No severe graft versus host disease was observed. One patient died of viral pneumonia 23 months post transplantation. One case is alive and has had a good health performance during the follow-up of 21 months. Conclusion The data here suggest that haplo-identical HSCT with umbilical cord mesenchymal stromal cell might provide an opportunity of salvage therapy for aplastic anemia with graft rejection after HLA-matched unrelated donor transplant.
Keywords：Haploidentical hematopoietic stem cell transplantationMesenchymal stem cellAplastic anemiaRejection
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 86-89 )