Engineered hepatic tissue based on newborn rat liver cells improved liver fibrosis in rats by orthotopic injection
ZHOU Hai-yang
ZHENG Ai-min
HUANG Rong-rong
DAI Da-jiang
ZHANG Hai-hong
CAO Hou-jun
Abstract：Objective To observe the effecting on the content of liver function in serum and liver fibrosis index of experimental liver fibrogenesis rats,and investigate wether injection of hepatic constructs based on neonatal rat is provided with anti-liver fibrogenesis effect. Methods The rats were divided into two groups,and in group one,engineered hepatic grafts were implanted by orthotopically multi-spot injection. Liver cells isolated from from neonatal SD rats were mixed with liquid collagen prepared from SD rat tails.While group two received the same volume of saline solution.Liver function was evaluated by serum biological analysis at 10days. After 20days rats were killed,fibrosis index was examined by HE staining and Sirius red staining of liver tissue. Results Compared to controls,injection of hepatic constructs based on neonatal rat improve the liver function. Moreover,fibrosis index got more improvement in cell-matrix mixture than that in control group. Conclusion Multi-site injection of hepatic constructs based on neonatal rat liver cells in the liver can protect against experimental liver fibrosis in CCl4-induced rats and the mechanisms will be studied further.
Keywords：Neonatal rat liver cellsLiver tissue engineeringLiver fibrosisOrthotopically injection
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 149-151,166 )