Analysis of cancer stem like cell characteristic in MKN-45 side population
ZHANG Hai-hong
WEI Xue-ming
FAN Qin
WANG Jian-mei
WANG Xue-jing
LI Li
SHI Wei
YUAN Xiao-ying
CHEN Lin
Abstract： Objective We tried to ascertain gastric cancer cell line MKN-45 contain SP cells, and learned about wether gastric cancer SP cells possess cancer stem-like characteristic. Method We used fluorescence activated cell sorting (FACS) to sort SP cells in human gastric carcinoma (GC) cell line MKN-45 cells labeled with Hoechst 33342, and then characterized the cancer stem-like properties of SP cells. Result The SP cells had higher clone formation efficiency than major population (MP) cells. Five stemness–related genes expression profiles, including OCT-4, SOX-2, NANOG, CD44 and ATP-binding cassette transporters gene ABCG-2, were tested in SP and MP cells using quantitative real-time RT-PCR. Western blot was chosen to show the difference of protein expression between SP and MP cells. Both results showed that there was significantly higher expression in SP cells than in MP cells. When inoculated into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice, SP cells showed higher tumorigenesis tendency than MP cells. Conclusion SP cells possess cancer stem cell properties and proved that SP cells from MKN-45 were gastric cancer stem-like cells.
Keywords：ATP-Binding cassette transportersSide-population cellsBenzimidazoles (hoe 33342)Stomach neoplasmStem cells
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 36-40 )