The Study on the Effect and Mechanism of miR-363-3p on the Biological Behavior of Laryngeal Squamous Cell Carcinoma
LIU Hao
YANG Ruiming
WU Yilin
LIU Mingyu
GONG Liang
Abstract：Objective To investigate the effects of miR-363-3p on the biological behavior of laryngeal cancer TU686 cells,and to analyze the target genes and mechanism of miR-363-3p.Methods A laryngeal cancer model overexpressing miR-363-3p was constructed through in vitro transfection;the effects of miR-363-3p on the functional status of laryngeal cancer TU686 cells were detected by CCK-8,Transwell experiments,and flow cytometry;the downstream target gene HIPK3 of miR-363-3p was predicted using the Targetscan bioinformatics database,and the double luciferase experiment was adopted for verification;RT-qPCR and Western Blot were used to detect the expression of HIPK3 in each group of cells after miR-363-3p overexpression.Results After overexpression of miR-363-3p,the proliferation rate of laryngeal cancer cells increased(P＜0.05),the invasion rate increased(P＜0.001),and the number of apoptotic cells decreased significantly(P＜0.001).The double luciferase experiment confirmed the targeting relationship between miR-363-3p and HIPK3,and the results of RT-qPCR and Western Blot experiments showed that the expression level of HIPK3 in the overexpression group decreased(P＜0.05).Conclusion miR-363-3p can promote the proliferation and invasion ability of laryngeal cancer TU686 cells,inhibit the apoptosis of laryngeal cancer TU686 cells,and there is a targeted regulatory relationship between miR-363-3p and HIPK3,promoting the occurrence and development of laryngeal cancer TU686 cells.
Keywords：laryngeal squamous cell carcinomamiR-363-3pHIPK3cancer cells
Publication Date：2026-02-28
Online Publishing Date：2026-03-20(First online date of this platform, not the publication date of the document)
Pages：7( 21-26,34 )