Study on Preparation and Anxiolytic Effect of Proteolytic Hydrolysates from Semen Ziziphi Spinosae
TIAN Li
WU Xiaolan
LIU Wenyan
Abstract：Objective To improve the extraction process of Semen Ziziphi Spinosae protein hydrolysates(SZPHs)using Semen Ziziphi Spinosae as raw material and study their biological activities.Methods Defatted Semen Ziziphi Spinosae powder was used to obtain Semen Ziziphi Spinosae protein by alkali extraction and acid precipitation.Alkaline protease and neutral protease were used to hydrolyze Semen Ziziphi Spinosae protein at different ratios.The 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging capacity of SZPHs was compared to screen the optimal protease for Semen Ziziphi Spinosae.The anti-anxiety effect of SZPHs was verified by mouse elevated plus maze test(EPM),light-dark box test,and serum glutamate(Glu)level.Results The DPPH scavenging capacity of SZPHs was significantly higher than that of Semen Ziziphi Spinosae protein.Among them,the hydrolysate of alkaline protease showed the strongest activity,with a DPPH free radical scavenging rate of up to 87.40%,indicating that SZPHs have antioxidant activity.SZPHs could increase the total arm entries,open time percentage(OT%),open arm entries percentage(OE%)in EPM,and total shuttle times in the light-dark box test(P＜0.01),while reducing the serum Glu content(P＜0.01).Conclusion SZPHs have antioxidant and anti-anxiety activities,showing broad prospects in the food industry.
Keywords：Semen Ziziphi Spinosaeprotein extractionproteolytic productsanti-anxiety activityantioxidant activity
Publication Date：2026-02-28
Online Publishing Date：2026-03-20(First online date of this platform, not the publication date of the document)
Pages：6( 8-13 )