Study on the Preparation Process of Total Flavonoids Liposomes from Suaeda salsa by Ethanol Injection Method and Its Anti-tumor Activity
LIU Ying
LIU Xinxin
YANG Qingshan
Abstract：Objective To optimize the preparation process of total flavonoids liposomes from Suaeda salsa and analyze their anti-tumor activity.Methods Ethanol injection was used to prepare liposomes of total flavonoids of Suaeda salsa.Single factor experiments were conducted to investigate the factors of lipid drug ratio,membrane material ratio,injection rate,and buffer PBS temperature.The encapsulation efficiency of liposomes was used as the index,and the preparation process was optimized by Box Behnken response surface methodology,the antitumor activity of total flavonoids liposomes from Suaeda salsa was evaluated using the MTT method.Results The optimal preparation formula for the total flavonoid liposomes of Suaeda salsa was a lipid drug ratio of 9.6∶1,a membrane material ratio of 7.6∶1,an injection rate of 0.8 mL/min,and a buffer PBS temperature of 50 ℃,the encapsulation efficiency of 82.03％.In vitro release experiments showed a cumulative release rate of 66.42％within 24 hours.The total flavonoid liposomes of Suaeda salsa had inhibitory effects on the proliferation of both rectal cancer HCT116 and HT29 cells.Conclusion The liposomes of total flavonoids of Suaeda salsa prepared by ethanol injection method are reliable and convenient,and the optimized liposomes have good sustained release and anti-tumor activity.
Keywords：Suaeda salsatotal flavonoidsliposomesresponse surfaceanti-tumor
Publication Date：2025-12-30
Online Publishing Date：2026-04-02(First online date of this platform, not the publication date of the document)
Pages：7( 90-96 )