Study on LncRNA XIST Promoting Proliferation of Colorectal Cancer Cells by Competitively Binding to miR-497-5p and Targeting EIF5A

HE Weiwei; LI Yingjie

doi:10.3969/j.issn.2096-305X.2025.06.005

Abstract：Objective To explore the role of lncRNA XIST in colon cancer and analyze whether it regulates the proliferation by competitively binding to microRNA-497-5p(miR-497-5p)targeting EIF5A,in order to provide ideas for exploring the mechanism of colon cancer.Methods Bioinformatics analysis was used to predict the expression trend of EIF5A in colon cancer and predict possible related target genes.Colon cancer SW480 cell line was selected,plasmids were constructed,and pc-DNA-XIST group,si-XIST group,miR-497-5p mimic group,miR-497-5p inhibitor group and si-EIF5A group were established respectively.Empty vector transfection(EV)group was also set up.Experimental methods such as CCK-8,qRT-PCR and dual luciferase reporter gene were applied,and rescue experiments were performed.Forty-seven patients undergoing colon cancer surgery were selected.Tumor tissues were collected as the observation group,and normal mucosal tissues were collected as the control group.The expression of LncRNA XIST and miR-497-5p was detected by qRT-PCR,and the expression of EIF5A was detected by immunohistochemistry.Results Bioinformatics analysis predicted that LncRNA XIST had a high expression trend in colon cancer.LncRNA XIST and miR-497-5p,miR-497-5p and EIF5A had binding base sequences.Compared with the EV group,the cell proliferation activity of the si-XIST group,miR-497-5p mimic group and si-EIF5A group was significantly decreased,and the cell proliferation activity of the pc-DNA-XIST group and miR-497-5p inhibitor group was significantly increased.The dual luciferase reporter gene experiment showed that LncRNA XIST and miR-497-5p,miR-497-5p and EIF5A had a targeting relationship.Rescue experiments showed that inhibition of miR-497-5p could partially reverse the inhibitory effects of silencing LncRNA XIST and silencing EIF5A on colon cancer cell proliferation.LncRNA XIST and EIF5A expression were increased in colon adenocarcinoma,while miR-497-5p expression was downregulated.Conclusion LncRNA XIST plays a promoting role in the formation and progression of colon cancer.LncRNA XIST promotes the proliferation of colon cancer cells by competitively binding to miR-497-5p and targeting EIF5A.