The Expression and Significance of CEACAM6,VEGF,ANG2,and miR-146a-5p in Prostate Adenocarcinoma

ZHOU Yang; JIN Yanyang

doi:10.3969/j.issn.2096-305X.2025.05.005

Abstract：Objective To investigate the expression levels of carcinoembryonic antigen associated cell adhesion molecular-6(CEACAM6),vascular endothelial growth factor(VEGF),angiopoietin 2(ANG2),and microRNA-146a-5p(miR-146a-5p)in prostate adenocarcinoma,and analyze their relationship with clinical pathological characteristics.Methods 77 patients diagnosed with prostate adenocarcinoma through pathology were selected as the research group,and their postoperative wax block tissues were collected;normal mucosal tissues at the edge of the tumor were retained as the control group.The immunohistochemical two-step method was used to detect CEACAM6,VEGF,and ANG2 in the tissues of both groups.RT-qPCR was applied to detect the expression level of miR-146a-5p in prostate cancer tissues,and the correlations between the related indicators and the clinical pathological characteristics of the patients(Gleason score,TNM stage,tumor grade,etc.)were analyzed.Results The positive expression rates of CEACAM6,VEGF and ANG2 proteins in the research group were significantly higher than those in the control group.The expressions of CEACAM6,VEGF and ANG2 were closely related to clinical pathological features such as Gleason score,TNM stage,tumor grade and intratumoral vascular tumor thrombus.The expression level of miR-146a-5p in the research group was significantly lower than that in the control group,and it was negatively correlated with the expressions of CEACAM6 and VEGF.Conclusion CEACAM6,VEGF and ANG2 are highly expressed in prostate adenocarcinoma,and are associated with the invasiveness,metastasis risk and poor prognosis of the tumor.miR-146a-5p may act as a tumor suppressor,influencing the progression of the tumor by regulating the expression of CEACAM6 and VEGF.