Alisma Orientalis Extract and Its Compound Prescription Reduce Body Weight and Body Fat in Obese Rats

ZHAO Yuxin; ZHAO Wenyi; SANG Xiaoyu; WU Liang; SUN Dongdong

doi:10.3969/j.issn.2096-305X.2025.05.004

Abstract：Objective To investigate the lipid-lowering effects of alisma orientale extract(AE)and its compound preparation(CP),which contains konjac flour,L-carnitine,and tea polyphenols,on obese rats fed with high-fat diet.Methods 80 rats were randomly divided into four groups:the blank group(fed with normal diet),the model group(fed with high-fat diet),the AE group,and the CP group(20 rats in each group).The intervention was carried out by intragastric administration for 4 weeks until 16 weeks.Body weight and food intake were monitored,and serum lipid metabolism indicators such as total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C),as well as liver function indicators such as alanine aminotransferase(ALT),aspartate aminotransferase(AST),were detected.To determine the oxidative stress level in liver tissue,the contents of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and malondialdehyde(MDA)were measured.At the same time,to evaluate the inflammatory response,the levels of tumor necrosis factor-α(TNF-α),interleukin-1 β(IL-1β),and interleukin-6(IL-6)were measured.Results After the intervention,the AE group and the CP group could reduce the weight increase,liver weight and liver index compared with the model group(P＜0.05),among which the CP group had a better effect.The serum levels of TG,TC,ALT,AST,and LDL-C in both intervention groups decreased by 17.30％to 28.10％,while HDL-C increased by 21.50％to 24.80％.The activities of SOD and GSH-Px in liver tissue increased by 34.70％to 41.20％,and the MDA content decreased by 39.40％to 45.10％.The levels of inflammatory factors TNF-α,IL-1β,and IL-6 decreased by 32.80％to 50.30％.Conclusion The extract of Alisma and its compound preparations can effectively inhibit lipid deposition in obese rats.The compound formulation has a more significant advantage in improving lipid metabolism disorders,reducing oxidative stress and inflammatory responses through a synergistic enhancement mechanism.