Simultaneous Detection of Blood Drug Concentrations of Remdesivir and Ritonavir by HPLC and Their Clinical Application

LI Xue

doi:10.3969/j.issn.2096-305X.2025.01.007

Abstract：Objective To establish a high performance liquid chromatography(HPLC)analysis method for the detection of remdesivir and ritonavir in human plasma samples.Methods In this experiment,the samples were analyzed by gradient elution method in high performance liquid chromatography.The chromatographic column of Elite Hypersil BDS C18 was selected,the size was 4.6 mm×250 mm,and the particle size was 5 μm.The column was equipped with an ultraviolet detector to detect the elution compounds using the absorption properties of the substances to ultraviolet light.The mobile phase of the experiment was composed of acetonitrile and 0.1%formic acid water,the flow rate was set at 0.3 mL/min,the column temperature was maintained at 55℃,the injection volume was 10 μL,and the blood concentrations of nematovir and ritonavir were quantitatively detected.Results The linear relationship of two components in human plasma samples was good within the range of 0.1-60 μg/mL(r>0.99),and the extraction recovery rates of low,medium,and high quality control plasma samples were 86.93%-97.10%.Both intra-day and inter-day precision,accuracy,and stability met the requirements for biological sample analysis.This method was applied to detect the drug concentrations of remdesivir and ritonavir in the plasma of 6 patients.The drug concentration of remdesivir was 0.69-0.81 μg/mL,and the drug concentration of ritonavir was 0.52-1.39 μg/mL.Conclusion The HPLC-UV quantitative analysis method established is rapid,accurate,and sensitive,and can be used for the determination of the content of remdesivir and ritonavir in human plasma.