Human Rhinovirus RV16 Promotes HMC-1 Mast Cell Activation

YIN Jiaqi; YANG Xinni; TAN Jingyu; LIU Haiwen

doi:10.3969/j.issn.2096-305X.2025.01.005

Abstract：Objective To investigate the effect of human rhinoviruses(HRV)infection on the activation of human mast cell line 1(MHC-1)and the function of immune response,and to explore the immunopathological mechanism of acute attack or aggravation of allergic asthma induced by HRV.Methods HMC-1 cells were randomly divided into three groups.Except for the RV-16virus infection group,UV inactivated RV-16 virus and PBS were used as negative control group and blank control group,respectively.Virus replication in HMC-1 cells and the expression of FcεR1,CD203c,CD63 were quantified by real-time quantitative PCR(qRT-PCR).The levels of tumor necrosis factor α(TNF-α),interleukin 6(IL-6),interleukin 8(IL-8)and interleukin 10(IL-10)secreted by HMC-1cells were detected by ELISA.Results After HRV infected HMC-1 cells,the viral load increased significantly at 24 hours(P<0.05),and reached the highest at 48 hours(P<0.01);Fluorescence quantitative PCR results showed that compared with control,the expression levels of activation-related molecules FcεR1,CD203c and CD63 in HMC-1 cells were significantly increased after RV16 virus infection(P<0.01);ELISA results showed that compared with the control group,the secretion of TNF-α,IL-6,IL-8 and IL-10 in HMC-1 cells increased significantly after RV16 virus infection(P<0.05).Conclusion HRV promotes the mast cell activation and enhances the cytokines secretion from mast cells,which is closely related to the exacerbation and pathogenesis of asthma caused by HRV.