Mechanism on the Improvement of Cognitive Dysfunction in Diabetic Mice by Carnosol

ZHANG Qiang; SHAN Wei; WANG Gang

doi:10.3969/j.issn.2096-305X.2025.01.002

Abstract：Objective To investigate the improvement of cognitive function in diabetic mice by Carnosol.Methods Construction of a diabetes model by intraperitoneal injection of streptozotocin in mice,and mice in the intervention group were given Carnosol gavage.Body weight and blood glucose of mice before modeling,3 d and 12 weeks of modeling were recorded;Morris Water Maze was used to assess the cognitive function in Diabetic Mice;Nissl staining was used to observe changes in the morphology and the number of neurons in hippocampal tissue;ELISA was used to for SOD activity and MDA content in mouse brain tissue;Western Blot was used to detect the protein expression levels of hippocampal CX3CL1,CX3CR1 proteins and oxidative stress SOD2.Results Blood glucose and weight results showed a significant increase in blood glucose and weight loss in the DM group at 12 weeks compared to the CON group(P<0.05),while the CL intervention improved these changes(P<0.05).The results of Morris Water Maze experiments showed that mice in the CL group had a significantly shorter escape latency and a significantly higher number of traversed platforms compared to the DM group(P<0.05).Nissl staining revealed an increase in the number of Nissl bodies and a significant change in neuronal morphology after CL intervention.Compared with the DM group,SOD activity was increased(P<0.05)and MDA content was decreased(P<0.05)in the brain tissue of mice in the CL treatment group.CX3CL1 and CX3CR1 protein expression were up-regulated(P<0.05,and SOD2 was significantly up-regulated(P<0.05).Conclusion Carnosol may alleviate oxidative stressand slow down neuronal damage in hippocampal tissues of DM mice through the CX3CL1/CX3CR1 pathway,which in turn improves cognitive function in DM mice.