Detection of in Vitro Metabolic Differences of Gastrodin in Liver Microsomes of Different Species by High Performance Liquid Chromatography
YAN Jie
Abstract：Objective To establish a high-performance liquid chromatography(HPLC)detection method for gastrodin in liver microsomes and identify its in vitro metabolic differences in different species.Methods Gastrodin liver microsome samples were treated by acetonitrile precipitation and separated by C18 chromatography column(4.6 mmx250 mm,5 µm)to establish HPLC method for gastrodin detection;gastrodin was incubated with different liver microsomes in phase Ⅰ(CYP)and phase Ⅱ(UGT)systems.The established HPLC method was used to detect the change of gastrodin content after incubation,and to determine the difference of its metabolism in different species.Molecular docking was applied to predict the interaction and sites between gastrodin and CYP450 enzymes.Results The precision,accuracy and stability of HPLC method for gastrodin liver microsomes met the requirements of biological sample analysis.There were significant differences in the metabolism of gastrodin among different species,and the metabolic characteristics of humans and monkeys were relatively similar.CYP3A4 and CYP2C19 may be the main metabolic enzymes of gastrodin,and gastrodin interacts with CYP through hydrogen bonding.Conclusion The metabolic differences of gastrodin among different species will provide a basis for the selection of experimental animals in future pharmacological and toxicological studies.
Keywords：gastrodinhigh-performance liquid chromatographyspecies differencemolecular docking
Publication Date：2024-10-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 34-38 )