The Potential Mechanisms of Potentilla Chinensis in Treating Gastric Cancer Based on Network Pharmacology and Bioinformatics Analysis

Li Pengmei; Li Xiaofei; Guo Lianyi

Abstract：Objective To explore the effective mechanisms of potentilla chinensis in treating gastric cancer utilizing network pharmacology and bioinformatics analysis.Methods The Traditional Chinese Medicine Database Analysis Platform(TCMSP)was employed within specific screening criteria to accurately detect the effective components in potentilla chinensis.The main active ingre-dient targets of potentilla chinensis were identified across various datasets including Swiss,SEA,TCMSP,PharmMapper,etc.The primary action targets of gastric cancer were obtained from three GSE files in the GEO database.The intersection of the drug targets predicted by the four databases and the gastric cancer targets predicted by the three GSE datasets yielded the common targets of poten-tilla chinensis and gastric cancer.A protein-protein interaction(PPI)network analysis was constructed using the STRING database to explore the relationship between potentilla chinensis and gastric cancer through their common targets.The common targets of potentilla chinensis and gastric cancer were subjected to GO and KEGG enrichment analysis using the Metascape platform,alongside visualiza-tion analysis through bioinformatics and visualization cloud platforms.Results 6 effective active ingredients and 748 target points were identified in potentilla chinensis through the TCMSP platform.833 gastric cancer targets were obtained from three GSE datasets,resul-ting in 49 common targets shared by potentilla chinensis and gastric cancer.The active components of potentilla in the treatment of gas-tric cancer were eugenol,ellagic acid,isorhamnetin,quercetin,kaempferol,β-sitosterol,etc.Corresponding targets included CX-CL8,SERPINE1,SPP1,CYP2C19,GSTA1 and so on,acting primarily through pathways like PI3K-Akt,MAPK,P53,etc.Conclusion The characteristics of potentilla chinensis in treating gastric cancer involve complexity in composition,rich target points,and diverse pathways.Applying network pharmacology theory and bioinformatics analysis,it is predicted that potentilla chinensis might exert its effects in treating gastric cancer through pathways like PI3K-Akt,MAPK,P53 and so on,offering theoretical insights for fu-ture exploration in gastric cancer research.