α-Mangostin Induces Apoptosis of Melanoma A375 Cells Through HMGB1 Proinflammatory Pathway

Ma Tingting; Wei Lingyan; Bao Dongyan

Abstract：Objective To investigate the effects of α-Mangostin on proliferation,migration and apoptosis of human melanoma A375 cells and the role of HMGB1-mediated inflammatory response during drug administration.Methods Human melanoma A375 cells were cultured with DMEM medium containing different drug concentrations(0,10,15 and 20 μM),and CCK-8 kit was used to detect cell viability and toxicity,and the morphological changes of cells were observed by optical microscopy;the migration ability of melanoma A375 cells in each group was detected by scratch assay;TUNEL staining was used to detect the apoptosis of melanoma A375 cells;cellular immunofluorescence assay was used to observe the expression of HMGB1 protein before and after treatment;Western Blot assay was used to detect the expression of HMGB1 and its associated inflammatory pathway proteins in human melanoma A375 cells treated with drugs.Results α-Mangostin was obviously toxic to A375 cells and inhibited proliferation and migration of A375 cells in a dose-dependent manner;TUNEL staining showed that α-twist significantly induced apoptosis of A375 cells;cellular immunofluorescence and Western Blot showed that the expression of HMGB1 and inflammation-related proteins in α-MG group was significantly decreased(P<0.01).Conclusion α-Mangostin can significantly inhibit the growth of human melanoma A375 cells,activate the HMGB1-related inflammatory pathway,and induce the apoptosis of human melanoma A375 cells.