Nine Kinds of Synthetic Pigments illegally Added in Salvia Miltiorrhiza Determined by HPLC

HU Lili; YANG Jingming; HU Shuang; YU Yanan

Abstract：Objective To develop a high performance liquid chromatography with multiple wavelength method for simultaneous determination of 9 kinds of yellow synthetic pigment(Tartrazize,Sunset yellow,Acid orange,etc.)in salvia miltiorrhiza.MethodsThe samples were extracted with ultrasonic from anhydrous ethanol: water (7∶3,v/v) and separated on TechMate ST-C18 column (4.6×250 mm,5 μm),by a gradient elution using methano (A) and 0.05 moL/L ammonium acetate(B) as mobile phase at flow rate of 1.0 mL/min, and the column temperature was 35 ℃.The wavelengths for detection were 405 nm(Acid yellow 17,Brillant yellow),430 nm(Tartrazize,Auramine O),485 nm(Sunset yellow,Acid orange 10,Basic orange 21, aurantia Ⅱand Basic orange 22).Results Under such chromatographic conditions,the specificity of the nine kinds of synthetic pigments was good,and there was no interference peaks.The calibration curves of 9 kinds of synthetic pigments showed good linear relationship in the range of 1.00~50.0 mg/L,r=0.999 9.The mean standard recovery rates ranged from 99.24% to 101.73%,RSD <2%.ConclusionThe method is simple,rapid,sensitive,stable,with high recovery rate and wide range of application.Therefore,it can be used for simultaneous determination of 9 kinds of synthetic pigments in salvia miltiorrhiza.