Relationship Between p16 Gene Methylation and HematogenousMicrometastasis in Non-small Cell Lung Cancer

WANG Wuming; LUO Daya; CHEN Xiaoqi; LIU Yuzhen

Abstract：Objective To explore the methylation status of p16 gene in non-small cell lung cancer and its correlation with blood micrometastasis.Methods Methylation-specific PCR (MSP) was used to analyze the methylation status of p16 gene in 60 patients with stage Ⅰ non-small cell lung cancer (NSCLC) and 60 patients with benign lung disease.Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect peripheral blood micrometastasis of non-small cell lung cancer patients.Results The detection rate of p16 gene methylation in non-small cell lung cancer tissues and benign lesions were 46.67% and 11.67%,respectively.The detection rate of p16 gene methylation in non-small cell lung cancer with hematogenous micrometastasis and without hematogenous micrometastasis were 62.96% and 33.33%,respectively.The detection rate of p16 gene methylation in lung cancer tissues was higher than that in benign lung tissues (P <0.05).The detection rate of p16 gene methylation in lung cancer patients with blood micrometastasis was higher than that without blood micrometastasis(P<0.05).There was no significant difference in the detection rate of p16 gene methylation in non-small cell lung cancer patients with different genders,ages,courses of disease,tumor sizes and histological types (P> 0.05).Conclusion The methylation rate of p16 gene in non-small cell lung cancer is higher,which is closely related to blood micrometastasis and may be involved in the occurrence and invasion of lung cancer.