3'RACE and 5'RACE Used to Clone Tryptophan Hydroxylase Gene in Central Nervous System of Nereis (N. japonica)
DONG Zhe
REN Guimin
LI Shen
YIN Haotian
BAO Xuexiang
WANG Shun
Abstract：Objective To Clone tryptophan hydroxylase gene in central nervous system of nereis ( N. japonica) , and provide evi-dence for the study of aromatic amino acid hydroxylase gene evolution in invertebrate. Methods Rapid amplification of cDNA ends ( RACE) was used to clone tryptophan hydroxylase gene. Results 3'sequence and 5'sequence of tryptophan hydroxylase gene were cloned in central nervous system of nereis ( N. japonica) . Conclusion Tryptophan hydroxylase gene exists in the central nervous sys-tem of Nereis ( N. japonica) independently, which carries forward the node of tryptophan hydroxylase and phenylalanine hydroxylase gene differentiation to annelid.
Keywords：RACEN. japonicatryptophan hydroxylasegene clone
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1-4 )