Effect of Xuebijing on Expression of TLR4-NF-kB Signaling Pathway Induced by LPS in Vascular Endothelial Cell
ZHANG Jia
HAN Bin
CHAI Wenshu
Abstract：Objective To investigate the effect of Xuebijing on the expression of TLR4-NF-kB signaling pathway induced by LpS in vascular endothelial cell. Methods Vascular endothelial cells in vitro culture were randomly divided into 3 groups, including con-trol group, LpS (1 mg/L) group, LpS (1mg/L) + XBJ (25 mg/mL) group. Western blot was used to detect the expression of TLR4 and NF-kB p65 protein. The concentration of inflammatory factor TNF-a was measured through ELISA. Results Compared with the control group, the concentration of TNF-a and the expression of TLR4 and NF-kB p65 protein in LpS group increased. Xue-bijing (25 mg/mL) decreased the concentration of TNF-a and the expression of TLR4 and NF-kB p65 protein (P<0. 01) significant-ly. Conclusions Xuebijing may inhibit inflammatory reaction and decrease the concentration of TNF-a protein through TLR4-NF-kB signaling pathway.
Keywords：XuebijingTLR4NF-kBendothelial cellsTNF-a
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 10-12 )