The Comparison of Different Molecular Biological Methods of Detecti ng the Samples of Hand-foot -an d -mouth Disease
CHEN Chuang
Abstract：Objective To explore the effects of different molecular biological methods of detecting the samples of hand-foot-and-mouth disease.Methods 120 samples of hand-foot-and-mouth disease in our hospital were collected and they were divided into re-al -time RT-PCRZ group, one-step RT-PCRZ group and nested PCR groupaccording to different molecularbiological methods.The sensitivity differences in detecting EV71 among these three groups were compared.Results The detection rates of real-time RT-PCR and nested PCR were significantly higher than that of one-step RT-PCR (P<0.05).Concluison In detection of hand-foot-and -mouth disease, real-time RT-PCR and nested PCR have higher detection accuracy, which are worthy of promotion in clinical detec-tion.
Keywords：hand-foot-and-mouth diseasePCRone-stepthe positive rate
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 21-22,29 )