Experimental Study of P53 Transfected Into Pancreatic Cancer Cells Mediated by High Intensity Focused Ultrasound Combined with Liposome

ZHANG Lansheng; ZHANG Linquan; HUA Daojin; ZHANG Tao; LI Caihong; ZHOU Hongping

Abstract：Objective To explore the feasibility of p53 transfected into pancreatic cancer cells mediated by high intensity focused ultrasound combined with liposome and the effect of the transfection on cell biological behaviour.Methods Pancreatic cancer cells were divided into 5 groups: simple cell group ( Group ) , HIFU-plasmid group ( Group II) , HIFU-liposome-plasmid group ( GroupШ) , liposome-plasmid group ( Group IV) , simple plasmid group ( Group V) .After proper treatment for the cells in the five groups, the mRNA expression of p53 in all the cells was detected with RT-PCR, and the proliferation and apoptosis of cells were detected with MTT assay and flow cytometry.Results With fluorescence microscopy, many cells with expression of green fluorescent protein were found in group III (with a transfection efficiency of 10.6%), a few in Group II and Group IV (with a transfection efficiency of 1.2%and 4.8%respectively), few in Group V (with a transfection efficiency of 0.3%), and none in Group I.The mRNA expression of p53 was found in Group III and Group IV, with significant difference in the grey level between the two groups (P<0.05).The results of MTT showed that the significant cell growth inhibition existed in Group II, Group III and Group IV, and the inhibition in Group III appeared the most notably with significant difference from other groups (P<0.05).The apoptosis rate in Group III was (14.2±1.55)%, significantly higher than that in other groups (P<0.05).Conclusions The transfection efficiency of p53 into pancreatic cells can be enhanced by HIFU combined with liposome.The transfection of p53 promotes cell apoptosis, further inhibiting the growth of tumor cells.