Effects of Cdc42 Small Interfering RNA onP roliferation of Breast Cancer MCF7 Cells
ZHAO Ning
ZHAO Lijie
Abstract：Objective To observe the effect of cell division cycle 42 (Cdc42) small interfering RNA (siRNA) on the prolifera-tion of breast cancer MCF7 cells.Methods The small interfering RNA in the targeted Cdc42 coding section was designed and synthe-sized, and transiently transfected into MCF7 cells.After 48 hours, the expressions of Cdc42 mRNA and protein after the transfection were detected with RT-PCR and Western blot respectively.The CCK8 as well as density experiments were applied to test the effect of Cdc42 siRNA on the reproductive capability of MCF7 cells.Results Both RT-PCR and Western blot showed that Cdc42 siRNA could notably lower the Cdc42 mRNA and its protein expression level in MCF7 cells.The proliferation of MCF7 cells was significantly inhibi-ted after the transfection of Cdc42 siRNA.Conclusion Cdc42 siRNA can significantly inhibit the proliferation of MCF7 cells, indica-ting that Cdc42 can be a promising target spot for treating breast cancer in the future.
Keywords：Cdc42MCF7RNA interferencecell reproduction
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 4-6 )