The Protective Effects of Pioglitazon on Rats with Focal Cerebral Ischemia-reperfusion Injury

ZHAO Defu

Abstract：Objective To observe the effects of PGZ on the expression of NF-κB, MMP-9 with focal cerebral ischemia-reperfu-sion and explore whether there’ s the inhibiting effect of PGZ for the damage of extracellular matrix. Methods 60 healthy rats are e-venly divided into 5 groups (n=12):the normal control group, the modal/ischemia-reperfusion group, the low dose of PGZ (10 mg ·kg-1 ) group, the middle dose of PGZ (15 mg·kg-1 ) group and the high dose of PGZ (20 mg·kg-1 ) group. After the correspond-ing processing, expressive difference of NF-κB and MMP-9 in Hippocampal tissue of rats can be analyzed by immune-histochemical and the western blot. Results Compared the model set with the normal control group, the neurological scores and the expression of NF-κB and MMP-9 of the ischemia-reperfusion group are increased (P<0. 01). The neurological scores and the expression of NF-κB and MMP-9 of the three different level dose PGZ groups are decreased in contrast to those of the ischemia-reperfusion group ( P<0. 01). The differences are obvious among the three different level dose PGZ groups. Conclusion The experiment indicates that PGZ can resist the damage of extracellular matrix through restraining the expression of NF-κB and MMP-9, alleviate the cerebral ischemia-reperfusion injury and play the role of brain protection.