Studies on Mechanisms of Rg3 Inducing Apoptosis through PI3 K/Akt Mediating MGBA in Breast Cancer Cells

PENG Mengjiao; ZHAI Hui; FENG Yanhua; XIN Laiwei; SUN Dapeng

Abstract：Objective To study whether ginsenoside Rg3 inhibits the expression of MGBA through PI3K/Akt signaling pathway and then promotes the apoptosis of breast cancer MDA-MB-231 cells. Methods Experimental groups:40 nmol/L IGF-1 group, 100 ng/mL Rg3 group, IGF-1+Rg3 group and control group. Western-blot tested the effect of Rg3 on the expression of p-Akt and MGBA in breast cancer MDA-MB-231cells. MTT assay tested the inhibition of proliferation of Rg3 on breast cancer MDA-MB-231cell after 48 hours. Flow cytometry assay tested the effect of Rg3 on apoptosis of breast cancer MDA-MB-231cells after 48 hours. Results Trans well test results show that Rg3 can inhibit the expression of MGBA in breast cancer MDA-MB-231cells, but the effect can be significantly inhibited when the p-Akt expression is enhanced; MTT assay test results show:compared with control group, IGF-1 group and IGF-1 + Rg3 group significantly promoted cell proliferation while Rg3 group significantly inhibited cell proliferation. Flow cytometry shows:compared with the control group, the apoptosis rates of IGF-1 group and IGF-1+ Rg3 group significantly de-crease while apoptosis rate in the Rg3 group is significantly enhanced. Conclusion It is through impacting on PI3K / Akt signaling pathway that ginsenoside Rg3 disturbs the expression of MGBA to promote the apoptosis of breast cancer cells.