Study of the Differentiation of Muscle-derived Stem Cells to Insulin-producing Cells Induced by Collagen Scaffold
LIU Rui
LIU Chang
YIN Tiantian
Abstract：Objective To study the possibility that the neonatal rat muscle-derived stem cells ( MDSCs) trans-differentiate to in-sulin-producing cells when collagen scaffold is added in culture medium and its possible mechanism. Methods MDSCs were extrac-ted and purified from rats’ skeletal muscles and identified with Desmin immunocytochemical staining. The MDSCs were then divided in-to 2 groups:collagen scaffold group and control group. The differentiation was induced and the cell morphology was observed. The in-sulin IPCs can be identified by dithizone ( DTZ) staining, and reverse transcription polymerase chain reaction ( RT PCR) was used to evaluate gene expression. Results Highly purified MDSCs were harvested and purified MDSCs were induced in these 2 groups. Ex-pressions of the precursor islet cells related genes Nestin, PDX-1 was detected by RT PCR in pancreas extract-induced group on the 6th day, and the typical islet like cell clusters were formed and insulin (INS) expression was detected on the 12th day after induction. Conclusions Chemical methods can induce differentiation of MDSCs to IPCs by simulating the three-dimensional microenvironment of islet cell development in Vitro.
Keywords：muscle-derived stem cellscollagen scaffoldinsulin secreting celltrans-differentiation
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 4-7,114 )