Effects and Mechanism of Hydrogen Sulfide on Ouabain-induced Arrhythmias
LIU Huixia
LI Lin
CHEN Baolang
YAN Changhong
WANG Xu
KONG Xiaoxia
LV Kun
Abstract：Objective To observe hydrogen sulfide′s effect on ouabain-induced arrhythmias and further explore its possible mech-anism. Methods Healthy Sprague-Dawley rats were used to establish a Langen-dorff retrograde perfusion system. Arrhythmia was e-licited in isolated rat hearts by the perfusion of 1 μmol/L Ouabain, sodium hydrosulfide (50, 100, 200 μmol/L) was given immedi-ately after the development of first sinus arrhythmia respectively. The arrhythmias were monitored and compared by simultaneous ECG recording, and the Na+-K+-ATP and Ca2+-Mg2+-ATP of myocardial cell in each group were examined. Results Ouabain could in-duce stable ventricular arrhythmias. Sodium hydrosulfide inhibited ouabain-induced premature ventricular beats ( PVB) in a concen-tration-dependent manner, and decreased the incidents of ventricular tachycardia (VT) and ventricular fibrillation (VF) (P<0. 05). Ouabain inhibited the activity of Na+-K+-ATP and Ca2+-Mg2+-ATP, while sodium hydrosulfide increased the activity. Conclusion Hydrogen Sulfide can perform anti-arrhythmic function and its mechanism may be related to the increasing activity of Na+-K+-ATP and Ca2+-Mg2+-ATP.
Keywords：hydrogen sulfideouabainarrhythmia
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 8-11 )