Effect of Endoplasmic Reticulum Stress Preconditioning on Bcl-2 and Bax Expression in Rats With Cerebral Ischemic-reperfusion
MIN Heming
JIA Yujie
CHENG Xue
BAO Cuifen
Abstract：Objective To explore the effect of preconditioning the endoplasmic reticulum stress induced by 2-DG on the neural cell apoptosis and the expression of related proteins, Bcl-2 and Bax, in the rats with cerebral ischemic-reperfusion. Method 24 SD rats were divided into 3 groups on average:the sham-operation group ( SH group) , ischemic-reperfusion group ( I/R group) , and en-doplasmic reticulum stress ( induced by 2-DG) preconditioning group ( ERS induced by 2-DG group) . The apoptotic cells in the hipp-ocampal field CA1 were tested with TUNEL, and the Bcl-2 and Bax protein expression changes in the same field were detected with the immunohistochemical method. Result The neurobehavioral scores and the apoptosis cell number significantly decreased in the ERS preconditioning group compared with those in the I/R group (P<0. 05). The cell number of Bcl-2 protein expression significantly in-creased (P<0. 01), while the cells with Bax protein expression significantly decreased (P<0. 01). Conclusion Endoplasmic reticu-lum stress preconditioning provides protection for cerebral ischemic-reperfusion injury by exerting its effect on Bcl-2 and Bax protein expression.
Keywords：endoplasmic reticulum stresspreconditioningcerebral ischemic reperfusionBcl-2Bax
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 5-7 )