Effect of Hypertonic Sodium Chloride Hydroxylethy Starch on HMGB1 and Apoptosis in Rats with Hemorrhagic Shock

ZHANG Lei; HOU Lixue; GUO Youxiang; OUYANG Jun

Abstract：Objective To investigate the effect of hypertonic saline-hydroxyethyl starch on ischemia-reperfusion injury of rats' intestine in hemorrhagic shock.Methods Rat models with hemorrhagic shock wereestablished in 60 SD rats by the method of Wig-ger’ s.The rats were divided into The noemal control group (N group), ringer resuscitation group (RL group), hydroxyethyl starch resuscitation group (HES group) and hypertonic saline (7.5%) combined with hydroxyethyl starch resuscitation group (HSH group), with 15 rats in each group.MAP at different time points were recorded, the ultrastructure in intestine tissue was observed by electron microscope, immunochemistry staining was used to detect the expression of HMGB1 on intestinal mucosa and the apoptosisof intestinal mucosa was tested.Results Pathological examination (HE staining) showed the HSH group and N group were significantly inferior in terms of protecting intestinal mucosal injury to RL group and HES group (P<0.01).HMGB1 detect showed the HSH group and N group were significantly lower than RL group and HES group (P<0.05).There was significant difference in apoptosis of intestinal mu-cosa in rats of RL, HES, N and HSH groups after hemorrhagic shock and resuscitation (P<0.05).Conclusion HSH can help to protect intestinal mucosa, suppress the expression of HMGB1 and reduce apoptosis, which would be a better way to protect the body cells and maintain organ function.