Analysis of Clinical Application of Polymerase Chain Reaction in Detection of Vaginal Bacteria

ZHOU Fawei

Abstract：Objective To study the clinical application of the polymerase chain reaction in detection of vaginal bacteria. Meth-ods Women with bacterial vaginosis were taken as the infected group (n=80), and the women who received health checkup at our hospital were taken as a control group ( n=81 ) , gardnerella in vaginal secretions was respectively detected by the method of fluores-cence quantitative polymerase chain reaction ( PCR) as well as Gram staining method, and then the detection results of gardnerella ob-tained by the two methods were compared. Results 80 cases from the infected group and 81 from the control group were sampled and detected by Gram staining, and the result showed that the positive rates were 73. 75% and 7. 4 %, respectively, the two groups were detected by PCR, and the result showed that the positive rates were 93. 75% and 8. 64% respectively. Gardnerella positive rates of the subjects of the infected group and the control group were detected by PCR fluorescence quantitative detection and Gram staining, the differences in detection results of these two methods were statistically significant (χ2=115. 7, P<0. 001, χ2=73. 6, P<0. 001). When the gardnerella of patients with bacterial vaginosis was detected, the comparison between the test results of PCR and Gram stai-ning showed that the positive rate of PCR detection was significantly higher than that of culture method (χ2=11. 76, P<0. 001). Con-clusion Real-time PCR method for detecting Gardnerella proves to be highly accurate, easy to operate and capable of meeting the needs of clinical diagnosis of bacterial vaginosis, and thus, it is worthy of being popularized and applied clinically.