Research on Antioxidant Activity in Vitro of Different Fractions from Total Flavonoids of Vitex negundo L. Seeds
ZHANG Xuhong
YANG Huiwen
PAN Yufang
Abstract：Objective To study the antioxidant activity in vitro of different fractions from total flavonoids of Vitex negundo L. seeds. Vitex negundo L. seeds were extracted and separated into four parts with different polarity. Method Ultrasonic extraction was employed to extract flavonoids ingredients with 70% ethanol. Different polars could be obtained by the extraction with chloroform, ethyl acetate and n-butanol. Three methods including ferric reducing power, total antioxidant power and 2, 2-diphenyl-1-picrylhyd-razyl radical scavenging assay were performed to detect the antioxidant activity, which was compared with that of vitamin C. Results The four different fractions of total flavonoids of vitex negundo L. seeds have different antioxidant activities, and there is good correlation between the antioxidant capacity and the density of flavonoids. Conclusion Among the four fractions, the chloroform layer exhibits the strongest antioxidant activities compared with ethyl acetate, n-butanol and water layer, and the iron reduction and total antioxidant forces of the chloroform layer are higher than VC, but its radical-scavenging activity is slightly lower than VC.
Keywords：vitex negundo L. seedstotal flavonoidsantioxidant activityVitamin C
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 4-6,9 )