Effect of Cadmium on Apoptosis of Mice Granulosa Cells

ZOU Xiaopan; ZHENG Xinyu

Abstract：Objective To study the effect of cadmium on apoptosis of mice granulosa cells and DNA integrity. Method Mice granulosa cells were exposed to cadmium ( Cd) by adding CdCl2 of different concentrations into the culture medium. Apoptosis and DNA integrity of granulosa cells was detected by using flow cytometry, DNA Ladder and trypan blue staining. Results Lower concen-tration (5 μM) CdCl2 treated cells for 24 h or higher concentration (20 μM and 50 μM) CdCl2 treated granulosa cells for 12 h could cause massive death of cells, flow cytometry results showed that with the increase of Cd concentration, the proportion of early apoptosis, late apoptosis, and dead cells was significantly increased after the treatment with different concentration of CdCl2 (P<0. 01), and mean-while, DNA fragmentation was also found in the process of apoptosis of granulosa cells induced by Cd. Conclusion 5μM concentration of cadmium can induce apoptosis of granulosa cells in mice, which might further affect the normal function of mouse ovaries.