The Expression and Clinical Significance of MiRNA-223, MiRNA-181a in Acute Leukemia

LIU Jia; RAN Xuehong

Abstract：Objective To investigate the expression of miRNA-223, miRNA-181a in acute leukemia (AL) and its prognostic value in AL.Methods The real-time quantitative analysis of variance ( ANOVA) was adopted to detect the expression of miRNA-223, miRNA-181a of 42 patients, including 35 cases with acute leukemia, 7 cases of acute lymphocytic leukemia, and 5 cases of non-leukemia patients at the same period in hospital as reference to analyze multiple sets of the sample mean by LSD of ANOVA.Results There were a total of 39 cases conforming to the standard in the collection of specimens ( including 29 AML patients , 6 ALL patients and 4 non-leukemia patients);the relative expression levels of miRNA-223, miRNA-181a in 29 AML patients were 6.65±1.29, 9.7 ±2.20 , and 4.76 ±0.76 , 10.06 ±2.47 in 6 ALL patients;the expression levels of miRNA-223 in AML and ALL were lower than those in normal control group ( P<0.01 ) , and the expression level of miRNA-223 in AML was significantly higher than that of ALL ( P<0.01 ) , the expression level of miRNA-181 a in AML and ALL were higher than those in normal control group ( P<0.01 );there was no significant difference of miRNA-181 a expression in AML and ALL;the expression level of miRNA-181 a in good prognosis group was higher than in middle prognosis group, the difference was statistically significant ( P<0.05 ) , not significantly different from APL group.In addition, the expression of miRNA-223 and miRNA-181a was not significantly different from the initial treatment group and the relapse group, the same in different age and gender.Conclusion The expression of miRNA-223, miRNA-181a was closely re-lated to AL, which can be used as a new standard in diagnosis of AL, especially significant in the differential diagnosis of AML and ALL, miRNA-181a may play an important role in the prognosis of AML, having a direct relationship with the long-term survival and the clinical efficacy;the expression of miRNA-223, miRNA-181a has nothing to do with age, gender, the initial treatment and the re-lapse.